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Downregulation of Bcl‐2 and activation of caspase‐8 in the UVB‐induced apoptosis of a cultured human melanoma cell line
Author(s) -
Park K. H.,
Choi H. O.,
Jang D. D.,
Park Y. I.,
Park K. C.
Publication year - 2001
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/j.1600-0781.2001.170503.x
Subject(s) - apoptosis , downregulation and upregulation , blot , melanoma , caspase , caspase 8 , fas ligand , cell culture , caspase 3 , uvb induced apoptosis , programmed cell death , chemistry , caspase 9 , microbiology and biotechnology , viability assay , cancer research , biology , biochemistry , genetics , gene
Purpose: This study was performed to determine the effect of UV radiation on the activation of apoptosis regulatory proteins using cultured human melanoma cells. Methods: G361 lightly pigmented melanoma cells were irradiated with increasing doses of UVB and analyzed for an apoptotic mechanism using a cell viability test, TEM, FACS, and western blotting analysis. Results: TEM and FACS showed apoptotic features of cell death after UVB irradiation. Western blotting disclosed downregulation of Bcl‐2 and the activation of caspase‐9. Caspase‐8, a downstream molecule of Fas/FasL interaction, was also activated. The activation of downstream molecules of both caspase‐8 and caspase‐9 was also demonstrated. Conclusion: Our data showed that the regulation of the Bcl‐2 family and caspase‐8 may work together to activate a caspase‐3 mediated apoptotic pathway following UVB irradiation of cultured human melanoma cells.