Premium
The antimicrobial effect of narrow‐band UVB (313 nm) and UVA1 (345–440 nm) radiation in vitro
Author(s) -
Fluhr J. W.,
Gloor M.
Publication year - 1997
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/j.1600-0781.1997.tb00230.x
Subject(s) - antimicrobial , in vitro , bacteria , irradiation , chemistry , microbiology and biotechnology , biology , physics , biochemistry , genetics , nuclear physics
Two new UV lamps were investigated with respect to their antibacterial effectiveness in vitro. Propioni ( n =20 strains) and Micrococcaceae ( n =16 strains) bacteria extracted from acne patients were applied to RCM and sheep blood agar plates and irradiated with a narrow‐band UVB lamp (TL 100W/10R) at a wavelength of 313 nm and a UVA1 lamp (TL 01) at a wavelength of 345–440 nm. The precisely defined energy levels were, in the case of narrow‐band UVB, 0.00, 0.30, 0.50, 1.00, 2.00 and 3.00 J/cm 2 and, in the case of UVA1, 0.00, 2.50, 5.00, 7.50, 10.00 and 20.00 J/cm 2 . UVA1 inhibited neither the growth of Propioni nor Micrococcaceae bacteria. In contrast, the growth of Micrococcaceae was inhibited already at a dosage of 0.30 J/cm 2 of narrow‐band UVB ( P <0.05), highly significant from 0.50 J/cm 2 ( P <0.01) and to a maximum of 2.2 powers of 10 at 3.00 J/cm 2 compared with non‐radiated control plates. Propioni bacteria were significantly inhibited at the minimum dosage of 0.30 J/cm 2 of narrow‐band UVB ( P <0.01) and to a maximum of 2.8 powers of 10 at 3.00 J/cm 2 .