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Photoprotective effect of calcipotriol upon skin photoreaction to UVA and UVB
Author(s) -
Youn J. I.,
Park B. S.,
Chung J. H.,
Lee J. H.
Publication year - 1997
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/j.1600-0781.1997.tb00126.x
Subject(s) - calcipotriol , erythema , dermatology , in vitro , keratinocyte , in vivo , calcitriol , medicine , viability assay , psoriasis , pharmacology , chemistry , cancer research , calcium , biology , biochemistry , genetics
It has been shown that 1,25‐dihydroxyvitamin D 3 has a photoprotective effect against UVB injury in mouse skin and cultured rat keratinocytes by induction of metallothionein (MT). Calcipotriol is a synthetic analogue of 1,25‐dihydroxyvitamin D 3 with equipotent cell regulating properties, but with a lower risk of calcium‐related side effects. The aim of the present study was to see whether calcipotriol has a photoprotective property both in vitro and in vivo. We examined the effect of calcipotriol on UV‐induced damage of cultured human keratinocytes through a cell viability assay, and measurement of DNA synthesis by cultured keratinocytes, on UV‐induced damage of mouse skin and on minimal erythema dose (MED). We found that calcipotriol was protective against UVB‐induced reduction in DNA synthetic activity of cultured keratinocytes in relatively low doses (20 and 40 mJ/cm 2 ) of UVB. With phototesting following application of calcipotriol, five subjects among 10 healthy volunteers and three among six psoriasis patients showed an increase in MED compared with the vehicle‐treated site. These findings imply that calcipotriol may be photoprotective and that more extensive studies with various doses of UV irradiation and modes of calcipotriol delivery are required.