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Psoralen‐fatty acid adducts activate melanocyte protein kinase C: a proposed mechanism for melanogenesis induced by 8‐methoxypsoralen and ultraviolet A light
Author(s) -
Anthony F. A.,
Laboda H. M.,
Costlow M. E.
Publication year - 1997
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/j.1600-0781.1997.tb00101.x
Subject(s) - diacylglycerol kinase , protein kinase a , biochemistry , protein kinase c , melanocyte , psoralen , fatty acid , ultraviolet light , phospholipase a2 , kinase , chemistry , biology , enzyme , photochemistry , cancer research , dna , melanoma
Diacylglycerol, a protein kinase C activator, induces and enhances melanogenesis in vitro and in vivo , providing evidence that melanogenesis may be a protein kinase C‐mediated process. Melanogenesis is also induced by ultraviolet A radiation and potentiated by a combination of 8‐methoxypsoralen and ultraviolet A radiation. We incubated cultured normal human melanocytes with 8‐methoxypsoralen, irradiated the cells with ultraviolet A radiation, and detected formation of 8‐methoxypsoralen‐phospholipid photoadducts. The 8‐methoxypsoralen‐phospholipid photoadducts isolated from melanocytes were substrates for phospholipase A 2 to generate 8‐methoxypsoralen‐fatty acid adducts. We found that 8‐methoxypsoralen‐fatty acid photoadducts prepared in vitro could be substituted for diacylglycerol to activate protein kinase C in a cell‐free system. We propose that 8‐methoxypsoralen‐fatty acid adducts activate protein kinase C to potentiate ultraviolet A radiation‐induced melanogenesis. This proposal links melanogenesis mediated by protein kinase C with that induced by a combination of 8‐methoxypsoralen and ultraviolet A radiation.