Premium
Interleukin‐15 expression by human endothelial cells: up‐regulation by ultraviolet B and psoralen plus ultraviolet A treatment
Author(s) -
Mohamadzadeh M.,
McGuire M. J.,
Dougherty I.,
Cruz P. D.
Publication year - 1996
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/j.1600-0781.1996.tb00238.x
Subject(s) - umbilical vein , dermis , downregulation and upregulation , microbiology and biotechnology , ultraviolet a , autocrine signalling , psoralen , interleukin 8 , interleukin , cytokine , ultraviolet b , biology , messenger rna , immunology , chemistry , cell culture , medicine , dermatology , dna , in vitro , biochemistry , anatomy , genetics , gene
The purposes of the present study were to determine whether endothelial cells express IL‐15 and to evaluate effects of ultraviolet B (UVB) and 8‐methoxypsoralens plus UVA (PUVA) on such expression. Cultured human endothelial cells derived from dermis or umbilical veins were subjected to reverse transcriptase‐polymerase chain reaction (RT‐PCR) and immunoblot analyses for the detection of IL‐15 mRNA and protein, respectively. Both dermal and umbilical vein endothelial cells were shown to express IL‐15 mRNA and protein, and these markers were upregulated following UVB or PUVA treatment (but not by UVA or 8‐methoxypsoralens alone). Also using RT‐PCR, dermal and umbilical vein endothelial cells were shown to express IL‐2Rγc mRNA. These results expand the sources of IL‐15 in skin to include keratinocytes, dermal fibroblasts, and now endothelial cells. That IL‐15 from all three skin cells can be upregulated by UV treatment suggests a role for this cytokine in photosensitive disorders. Finally, the possibility of an autocrine effect of IL‐15 on endothelial cells is raised by the expression of IL‐2Rγc in these cells.