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In vitro photoinhibition by psoralen and ultraviolet A radiation of human hematopoietic progenitors
Author(s) -
Procaccini E.M.,
Selleri C.,
Monfrecola G.,
Camera A.,
Villa M. R.,
Notaro R.,
Grazia C.,
Posteraro G.,
Rotoli B.
Publication year - 1996
Publication title -
photodermatology, photoimmunology and photomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.736
H-Index - 60
eISSN - 1600-0781
pISSN - 0905-4383
DOI - 10.1111/j.1600-0781.1996.tb00200.x
Subject(s) - clonogenic assay , haematopoiesis , progenitor cell , bone marrow , psoralen , in vitro , peripheral blood mononuclear cell , immunology , puva therapy , microbiology and biotechnology , myeloid , biology , chemistry , cancer research , stem cell , biochemistry , dna , psoriasis
The in vitro sensitivity of human hematopoietic progenitors to PUVA, 8‐MOP and UVA alone was investigated. 8‐MOP alone at final concentrations of 150, 200, 600 and 1000 ng/ml did not modify colony growth of circulating and bone marrow erythroid (BFU‐E), myeloid (CFU‐GM) and immature (CFU‐GEMM) hematopoietic progenitors obtained from normal controls. The exposure of the same progenitors to increasing doses of UVA, up to 12 J/cm 2 , progressively decreased hematopoietic colony growth (with estimated 50% inhibition occurring at about 5 J/cm 2 ). In vitro PUVA treatment (8‐MOP 200 ng/ml followed by UVA 5 J/cm 2 ) caused 90% growth inhibition of circulating and bone marrow hematopoietic progenitors. In addition, the treatment completely inhibited the formation of spontaneous erythroid colonies, obtained from 5 polycythemic patients, that are considered to be a marker of this neoplastic disease. PUVA cytotoxicity was assessed by the colorimetric MTT assay. The percentage of cell death after PUVA exposure was 29 ± 10% for both peripheral and bone marrow mononuclear cells. Our findings indicate that 8‐MOP alone is not toxic to hematopoietic progenitors whereas UVA treatment determines in vitro a dose‐dependent inhibition of the clonogenic capacity of normal hematopoietic cells. PUVA treatment enhances this effect, causing a quite complete inhibition of hematopoietic progenitors colony formation from normal donors and spontaneous BFU‐E colony formation from polycythemic patients.