Open AccessCytoprotection by Deprenyl and Tolcapone in a Cell Culture Model of Cerebral Ischaemia
Author(s) -
Ekblom Jonas,
Tottmar Olof,
Oreland Lars
Publication year - 1998
Publication title -
pharmacology & toxicology
Language(s) - English
Resource type - Journals
eISSN - 1600-0773
pISSN - 0901-9928
DOI - 10.1111/j.1600-0773.1998.tb01468.x
Subject(s) - lactate dehydrogenase , ischemia , cerebral ischaemia , cytoprotection , pharmacology , cell damage , dehydrogenase , brain cell , chemistry , medicine , enzyme , biochemistry , biology , apoptosis , microbiology and biotechnology
Abstract: Foetal rat brain aggregation cultures were exposed to a single episode of anoxia and hypoglycaemia for 30 min. Lactate dehydrogenase specific activity was estimated in the culture medium after ischaemia as a marker of lost cell integrity. Release of lactate dehydrogenase was most prominent during the first 24 hr period after the ischaemic damage, then it gradually declined. Immediately after ischaemic exposure, the cultures were treated with different concentrations of L‐deprenyl or tolcapone. Significantly lower amounts of lactate dehydrogenase leaked into the culture medium during the first 24 hr after the ischaemic episode in cultures treated with deprenyl or tolcapone (1–100 nM). These results suggest that deprenyl and tolcapone may reduce cell damage after ischaemia, at doses causing enzyme inhibition.