Open AccessThe Effect of 1,25‐Vitamin D 3 on Calbindin‐D and Calcium‐Metabolic Variables in the Rat
Author(s) -
Hemmingsen Claus,
Staun Michael,
Olgaard Klaus
Publication year - 1998
Publication title -
pharmacology & toxicology
Language(s) - English
Resource type - Journals
eISSN - 1600-0773
pISSN - 0901-9928
DOI - 10.1111/j.1600-0773.1998.tb01409.x
Subject(s) - medicine , endocrinology , calbindin , calcium , vitamin d and neurology , vitamin d dependent calcium binding protein , transcellular , calcium metabolism , chemistry , vitamin , biology , biochemistry
Intraperitoneal injection of l,25‐(OH) 2 D 3 4 μg/kg was given to 84 calcium‐ and vitamin D‐repleted Wistar rats and samples of plasma, duodenal mucosa and renal tissue were taken after 0, 3, 6, 12, 24, 48 and 96 hr (n = 12 at each time interval). Plasma‐ionized Ca increased after 6 hr, reached a maximum after 24 hr and returned to the initial values after 96 hr. The concentrations of renal ealbindin‐D28k and intestinal calbindin‐D9k did not increase until 48 hr after injection and remained elevated until 96 hr after. Therefore, significantly elevated concentrations of the cytosolic calbindinD were found at a time with normal values of plasma Ca. The present data suggest that calbindin‐D does not alone increase the transcellular Ca transport and, therefore, supports the view that calbindin‐Ds may serve as Ca buffer proteins.