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Oral mucosa model based on a collagen–elastin matrix
Author(s) -
Golinski P. A.,
Gröger S.,
Herrmann J. M.,
Bernd A.,
Meyle J.
Publication year - 2011
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.2011.01393.x
Subject(s) - elastin , oral mucosa , ex vivo , tissue engineering , scaffold , basement membrane , biocompatibility , pathology , chemistry , matrix (chemical analysis) , biomedical engineering , materials science , medicine , in vitro , biochemistry , organic chemistry , chromatography
Golinski PA, Gröger S, Herrmann JM, Bernd A, Meyle J. Oral mucosa model based on a collagen–elastin matrix. J Periodont Res 2011; 46: 704–711. © 2011 John Wiley & Sons A/S Background and Objective: The collagen‐elastin matrix (Matriderm ® ) is used to treat deep and full‐thickness burns and was recently described as a suitable scaffold for tissue engineering. The aim of the present study was to investigate the biocompatibility of Matriderm ® for gingival use through creation of an oral mucosa model ex vivo . Material and Methods: Gingival fibroblasts and keratinocytes were cultured. A dermal area on the base of the collagen–elastin matrix was repopulated with fibroblasts. After 14 days, keratinocytes were seeded on this dermal area to engineer a multilayered mucosa. Analysis of the architecture was performed using light and electron microscopy. Immunohistochemical detection of collagen IV and cytokeratin was carried out. Results: Based on this scaffold we generated a multilayered oral mucosa‐like structure. Histological, immunohistochemical and electron microscopic analysis of the dermal/epidermal junction showed a typical basement membrane and hemidesmosomal structures. Neighboring keratinocytes formed desmosomes in the epidermal sections. Cytokeratin was detectable in all epidermal layers. These experiments revealed that the collagen–elastin matrix was highly biocompatible with gingival cells under ex vivo conditions. Conclusion: Employing tissue‐engineering techniques with dermal and epidermal cells from the gingiva, a multilayered oral mucosa was generated and characterized with respect to biocompatibility for Matriderm ® . The results indicate that Matriderm ® is suitable for the ex vivo growth of gingival tissue cells and is a useful scaffold with possible applications in periodontal therapy.