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Effects of adenoviral‐mediated coexpression of bone morphogenetic protein‐7 and insulin‐like growth factor‐1 on human periodontal ligament cells
Author(s) -
Yang L.,
Zhang Y.,
Dong R.,
Peng L.,
Liu X.,
Wang Y.,
Cheng X.
Publication year - 2010
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.2009.01268.x
Subject(s) - periodontal fiber , bone morphogenetic protein 2 , bone morphogenetic protein , growth factor , runx2 , alkaline phosphatase , periodontal ligament stem cells , bone morphogenetic protein 7 , microbiology and biotechnology , cell growth , chemistry , biology , in vitro , medicine , dentistry , gene , biochemistry , receptor , enzyme
Yang L, Zhang Y, Dong R, Peng L, Liu X, Wang Y, Cheng X. Effects of adenoviral‐mediated coexpression of bone morphogenetic protein‐7 and insulin‐like growth factor‐1 on human periodontal ligament cells. J Periodont Res 2010; 45: 532–540. © 2010 John Wiley & Sons A/SBackground and Objective: Bone morphogenetic protein‐7 ( BMP‐7 ) and insulin‐like growth factor‐1 ( IGF‐1 ) are important in periodontal reconstruction. However, their synergistic effect in periodontal regeneration by gene delivery has not been reported. In this study, gene delivery of these two growth factors to human periodontal ligament cells (hPDLCs) was examined for its effects on cell proliferation and differentiation. Material and Methods: Recombinant adenoviruses containing both human BMP‐7 and IGF‐1 cDNA created by introducing the internal ribosome entry site (IRES) sequence were used to transfer the genes into hPDLCs. 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay and cell cycle analysis were used to observe their effects on cell proliferation, while alkaline phosphatase activity measurement, RT‐PCR and in vivo tests were conducted to investigate their effects on cell differentiation. Results: The proliferation of hPDLCs transduced by adenoviruses coexpressing BMP‐7 and IGF‐1 was suppressed while their differentiation ability was enhanced. There was a synergism of BMP‐7 and IGF‐1 in up‐regulating alkaline phosphatase activity and mRNA levels of collagen type I and Runx2. Implantation in vivo with scaffolds illustrated that the transduced cells exhibited osteogenic differentiation and formed bone‐like structures. Conclusion: The combined delivery of BMP‐7 and IGF‐1 genes using an IRES‐based strategy synergistically enhanced differentiation of hPDLCs. It is suggested that this could be a new potential method in gene therapy for periodontal reconstruction.