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Immunolocalization of laminin and integrin in regenerating junctional epithelium of mice after gingivectomy
Author(s) -
Masaoka T.,
Hashimoto S.,
Kinumatsu T.,
Muramatsu T.,
Jung H.S.,
Yamada S.,
Shimono M.
Publication year - 2009
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.2008.01142.x
Subject(s) - junctional epithelium , laminin , integrin , epithelium , basal lamina , connective tissue , extracellular matrix , biology , fibronectin , hemidesmosome , pathology , microbiology and biotechnology , chemistry , anatomy , cell , medicine , biochemistry , ultrastructure
Background and Objective: The expression patterns of adhesive proteins and extracellular matrix proteins in regenerating gingival epithelium after gingivectomy are unknown. The aim of this study was to examine the expression of laminin 1, laminin γ 2 (a specific component of laminin 5), integrin β 4 and integrin α 3 in the regenerating gingival epithelium in order to understand the mechanism of wound healing during reconstitution of the sulcular environment. Material and Methods: The palatal gingivae of the maxillary molars of Institute of Cancer Research mice were excised, and the regenerating tissues were examined 1, 3, 5, 7 and 14 days later. Fresh, non‐fixed and non‐decalcified frozen sections were prepared and stained using immunofluorescence. Results: At 1 day post‐surgery, intense expression of laminin γ 2 , integrin β 4 and integrin α 3 was distinct in the frontal margin of the regenerating oral epithelium. Laminin γ 2 was diffusely detected on the root surface and in connective tissues beneath the regenerating oral epithelium at 3 and 5 days. At 7 days, laminin γ 2 was intermittently recognizable in the internal basal lamina (IBL) close to tooth‐facing cells, while laminin γ 2 , integrin β 4 and integrin α 3 were observed in the IBL and in the external basal lamina (EBL) of the regenerating junctional epithelium at 14 days. Conclusion: These results suggest that secretion of laminin 5 in the connective tissue may induce epithelial cell migration, and that binding of laminin 5 to integrin α 6 β 4 and integrin α 3 β 1 in the IBL may provoke cell adhesion and migration of cells facing the tooth on the enamel surface of the regenerating junctional epithelium.