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Induction of cementogenesis and periodontal ligament regeneration by recombinant human transforming growth factor‐β3 in Matrigel with rectus abdominis responding cells
Author(s) -
Ripamonti U.,
Parak R.,
Petit J.C.
Publication year - 2009
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.2008.01086.x
Subject(s) - cementogenesis , periodontal fiber , cementum , regeneration (biology) , matrigel , furcation defect , anatomy , dental alveolus , endochondral ossification , growth factor , medicine , dentistry , cartilage , biology , microbiology and biotechnology , receptor , dentin , angiogenesis
Background and Objective: In primates and in primates only, the transforming growth factor‐β proteins induce endochondral bone formation. Transforming growth factor‐β3 also induces periodontal tissue regeneration. Two regenerative treatments using human recombinant transforming growth factor‐β3 were examined after implantation in mandibular furcation defects of the nonhuman primate, Papio ursinus . Material and Methods: Class III furcation defects were surgically created bilaterally in the mandibular first and second molars of two adult Chacma baboons ( P. ursinus ). Different doses of recombinant transforming growth factor‐β3 reconstituted with Matrigel ® matrix were implanted in the rectus abdominis muscle to induce heterotopic ossicles for subsequent transplantation to selected furcation defects. Twenty days after heterotopic implantation, periodontal defects were re‐exposed, further debrided and implanted with minced fragments of induced heterotopic ossicles. Contralateral class III furcation defects were implanted directly with recombinant transforming growth factor‐β3 in Matrigel ® matrix with the addition of minced fragments of autogenous rectus abdominis muscle. Treated quadrants were not subjected to oral hygiene procedures so as to study the effect of the direct application of the recombinant morphogen in Matrigel ® on periodontal healing. Histomorphometric analyses on undecalcified sections cut from specimen blocks harvested on day 60 measured the area of newly formed alveolar bone and the coronal extension of the newly formed cementum along the exposed root surfaces. Results: Morphometric analyses showed greater alveolar bone regeneration and cementogenesis in furcation defects implanted directly with 75 μg of transforming growth factor‐β3 in Matrigel ® matrix with the addition of minced muscle tissue. Conclusion: Matrigel ® matrix is an optimal delivery system for the osteogenic proteins of the transforming growth factor‐β superfamily, including the mammalian transforming growth factor‐β3 isoform. The addition of minced fragments of rectus abdominis muscle provides responding stem cells for further tissue induction and morphogenesis by the transforming growth factor‐β3 protein.