Premium
Naringenin has anti‐inflammatory properties in macrophage and ex vivo human whole‐blood models
Author(s) -
Bodet C.,
La V. D.,
Epifano F.,
Grenier D.
Publication year - 2008
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.2007.01055.x
Subject(s) - naringenin , lipopolysaccharide , aggregatibacter actinomycetemcomitans , tumor necrosis factor alpha , macrophage , periodontitis , immunology , cytokine , treponema denticola , ex vivo , interleukin , hesperetin , chemistry , microbiology and biotechnology , porphyromonas gingivalis , biology , medicine , biochemistry , in vitro , flavonoid , antioxidant
Background and Objective: Periodontitis is a chronic inflammatory disease of bacterial etiology, affecting tooth‐supporting tissues. The host inflammatory response to periodontopathogens, notably the high and continuous production of cytokines, is considered a major factor causing the local tissue destruction observed in periodontitis. The aim of the present study was to investigate the effect of naringenin, a major flavanone in grapefruits and tomatoes, on the lipopolysaccharide‐induced pro‐inflammatory cytokine production by host cells, using two different models. Material and methods: The effect of naringenin was characterized using macrophages stimulated with the lipopolysaccharide of either Aggregatibacter actinomycetemcomitans or Escherichia coli and using whole blood stimulated with A. actinomycetemcomitans lipopolysaccharide, in the presence or absence of naringenin. Lipopolysaccharide‐induced interleukin‐1β, interleukin‐6, interleukin‐8 and tumor necrosis factor‐α production by macrophages and whole‐blood samples treated with naringenin were evaluated using an enzyme‐linked immunosorbent assay. Changes in the phosphorylation states of macrophage kinases induced by A. actinomycetemcomitans lipopolysaccharide and naringenin were characterized by immunoblot screening. Results: Our results clearly indicated that naringenin is a potent inhibitor of the pro‐inflammatory cytokine response induced by lipopolysaccharide in both macrophages and in whole blood. Naringenin markedly inhibited the phosphorylation on serines 63 and 73 of Jun proto‐oncogene‐encoded AP‐1 transcription factor in lipopolysaccharide‐stimulated macrophages. Conclusion: The results from the present study suggest that naringenin holds promise as a therapeutic agent for treating inflammatory diseases such as periodontitis.