Premium
Hydrogen sulfide inhibits cell proliferation and induces cell cycle arrest via an elevated p21 Cip1 level in Ca9‐22 cells
Author(s) -
Takeuchi H.,
Setoguchi T.,
Machigashira M.,
Kanbara K.,
Izumi Y.
Publication year - 2008
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.2007.00999.x
Subject(s) - cell cycle , cell growth , microbiology and biotechnology , cell cycle checkpoint , chemistry , blot , cell , flow cytometry , hydrogen sulfide , dna synthesis , cell culture , biochemistry , biology , dna , sulfur , genetics , organic chemistry , gene
Background and Objective: Volatile sulfur compounds such as hydrogen sulfide (H 2 S) and methyl mercaptan (CH 3 SH) are the main causes of oral malodor. However, the physiological functions of H 2 S have not been investigated in oral tissues. The aim of this study was to evaluate the effect of H 2 S on cell proliferation and the cell cycle in oral epithelial‐like cells. Material and Methods: Ca9‐22 cells were used in this study. Cells were cultured in 5% CO 2 /95% air with (5 or 10 ng/mL) or without H 2 S. DNA synthesis was measured using a 5‐bromo‐2‐deoxyuridine enzyme‐linked immunosorbent assay. The cell cycle was analyzed using a flow cytometer. The expressions of phosphorylated retinoblastoma protein (Rb), p21 Cip1 and p27 Kip1 were evaluated by western blotting. Results: Exposure to 5 and 10 ng/mL of H 2 S significantly decreased DNA synthesis ( p < 0.05). Cell cycle analysis also showed that exposure to both concentrations of H 2 S significantly increased the proportion of cells in G 1 phase ( p < 0.001) and significantly decreased the proportion of cells in S phase ( p < 0.01). Western blotting showed that Rb phosphorylation was reduced and p21 Cip1 was enhanced by exposure to H 2 S. Conclusion: The results indicated that H 2 S inhibits cell proliferation and induces cell cycle arrest via the expression of p21 Cip1 in Ca9‐22 cells.