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Inhibitory effect of cranberry polyphenol on biofilm formation and cysteine proteases of Porphyromonas gingivalis
Author(s) -
Yamanaka A.,
Kouchi T.,
Kasai K.,
Kato T.,
Ishihara K.,
Okuda K.
Publication year - 2007
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.2007.00982.x
Subject(s) - porphyromonas gingivalis , chemistry , polyphenol , biofilm , fusobacterium nucleatum , bacteroidaceae , fraction (chemistry) , proteases , microbiology and biotechnology , food science , chromatography , biochemistry , enzyme , biology , bacteria , antioxidant , genetics
Background and Objective: The purpose of this study was to investigate the effects of cranberry polyphenol fraction on biofilm formation and activities of Arg‐gingipain and Lys‐gingipain in Porphyromonas gingivalis. Material and Methods: The polyphenol fraction was prepared by using a glass column packed with Amberlite XAD 7HP and 70% aqueous ethanol as an elution solvent. Results: Synergistic biofilm formation by P. gingivalis and Fusobacterium nucleatum was significantly inhibited by the polyphenol fraction at a concentration of 250 µg/mL compared with untreated controls ( p < 0.01). Arg‐gingipain and Lys‐gingipain activities in P. gingivalis ATCC 33277 and FDC 381 were inhibited significantly at a polyphenol fraction concentration of ≥ 1 µg/mL ( p < 0.05). Conclusion: These findings indicate that the polyphenol fraction inhibits biofilm formation and the Arg‐gingipain and Lys‐gingipain activities of P. gingivalis .