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Identification of marker genes distinguishing human periodontal ligament cells from human mesenchymal stem cells and human gingival fibroblasts
Author(s) -
Fujita T.,
Iwata T.,
Shiba H.,
Igarashi A.,
Hirata R.,
Takeda K.,
Mizuno N.,
Tsuji K.,
Kawaguchi H.,
Kato Y.,
Kurihara H.
Publication year - 2007
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.2006.00944.x
Subject(s) - mesenchymal stem cell , stem cell , periodontal fiber , biology , stem cell transplantation for articular cartilage repair , bone marrow , microbiology and biotechnology , immunology , adult stem cell , cellular differentiation , medicine , gene , genetics , dentistry
Background and Objective: Molecular gene markers, which can distinguish human bone marrow mesenchymal stem cells from human fibroblasts, have recently been reported. Messenger RNA levels of tissue factor pathway inhibitor‐2, major histocompatibility complex‐DR‐α, major histocompatibility complex‐DR‐β, and neuroserpin are higher in human bone marrow mesenchymal stem cells than in human fibroblasts. However, human bone marrow mesenchymal stem cells express less apolipoprotein D mRNA than human fibroblasts. Periodontal ligament cells are a heterogeneous cell population including fibroblasts, mesenchymal stem cells, and progenitor cells of osteoblasts or cementoblasts. The use of molecular markers that distinguish human bone marrow mesenchymal stem cells from human fibroblasts may provide insight into the characteristics of human periodontal ligament cells. In this study, we compared the molecular markers of human periodontal ligament cells with those of human bone marrow mesenchymal stem cells and human gingival fibroblasts. Material and Methods: The mRNA expression of the molecular gene markers was analyzed using real‐time polymerase chain reaction. Statistical differences were determined with the two‐sided Mann–Whitney U ‐test. Results: Messenger RNA levels of major histocompatibility complex‐DR‐α and major histocompatibility complex‐DR‐β were lower and higher, respectively, in human periodontal ligament cells than in human bone marrow mesenchymal stem cells or human gingival fibroblasts. Human periodontal ligament cells showed the lowest apolipoprotein D mRNA levels among the three types of cells. Conclusion: Human periodontal ligament cells may be distinguished from human bone marrow mesenchymal stem cells and human gingival fibroblasts by the genes for apolipoprotein D, major histocompatibility complex‐DR‐α, and major histocompatibility complex‐DR‐β.