Premium
Selective inhibition of Porphyromonas gingivalis growth by a factor Xa inhibitor, DX‐9065a
Author(s) -
Matsushita Kenji,
Imamura Takahisa,
Tancharoen Salunya,
Tatsuyama Syouko,
Tomikawa Munehiro,
Travis James,
Potempa Jan,
Torii Mitsuo,
Maruyama Ikuro
Publication year - 2006
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.2005.00854.x
Subject(s) - porphyromonas gingivalis , trypsin , bacteroidaceae , microbiology and biotechnology , prevotella intermedia , chemistry , periodontitis , virulence factor , bacteria , biology , enzyme , biochemistry , virulence , medicine , genetics , gene
Background: Porphyromonas gingivalis is a causative bacterium of adult periodontitis. However, there is no drug specific for P. gingivalis and for its virulence factor. Objectives: The objective of this study was to examine the effects of a new selective inhibitor of activated factor X, DX‐9065a, on growth of Porphyromonas gingivalis and other periodontopathic bacteria. Methods: We incubated P. gingivalis and other periodontopathic bacteria in the presence or absence of DX‐9065a and examined the effect of DX‐9065a on bacterial growth and trypsin‐like activity in its cultures. We also examined the effects of DX9065a on amidolytic activity of purified trypsin‐like proteinases (gingipains RgpA and RgpB), from P. gingivalis and on trypsin‐like activity in gingival crevicular fluids from patients with adult periodontitis. Results: DX‐9065a selectively inhibited the growth of P. gingivalis and Prevotella intermedia , and its effect on P. gingivalis was bactericidal. Trypsin‐like proteinase activity was detected in P. gingivalis, and the activity was strongly inhibited by DX‐9065a. DX‐9065a even inhibited amidolytic activity of RgpA and RgpB from P. gingivalis . Furthermore, trypsin‐like proteinase activity in gingival crevicular fluids was strongly inhibited by DX‐9065a. Conclusions: DX‐9065a inhibits P. gingivalis growth in part through to its ability to inhibit the trypsin‐like proteinase activity in P. gingivalis and may be useful for a new drug for treatment of adult periodontitis.