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A functional interleukin‐1β gene polymorphism is associated with chronic periodontitis in a sample of Brazilian individuals
Author(s) -
Moreira Paula R.,
De Sá Alessandra R.,
Xavier Guilherme M.,
Costa José E.,
Gomez Ricardo S.,
Gollob Kenneth J.,
Dutra Walderez O.
Publication year - 2005
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.2005.00801.x
Subject(s) - chronic periodontitis , genotype , allele , locus (genetics) , periodontitis , biology , polymerase chain reaction , gene polymorphism , genetics , gene , microbiology and biotechnology , medicine
Background: Interleukin‐1 beta (IL‐1β) is a potent inflammatory mediator and an important polymorphism in the locus +3954 (C/T) of the human IL1B gene has been shown to affect the levels of this cytokine. This functional polymorphism has been associated with the establishment of inflammatory diseases, including periodontal disease, in European, Asian and North American populations. Objective: The aim of this study was to investigate the association between the IL1B (+3954) gene polymorphism and the occurrence of different clinical forms of periodontitis in a sample of Brazilian individuals. Methods: This study employed a cross‐sectional design involving individuals from the State of Minas Gerais in the south‐eastern region of Brazil. Genomic DNA was obtained from oral swabs of 129 individuals and amplified using the polymerase chain reaction (PCR) with specific primers flanking the locus +3954 of IL1B . PCR products were submitted to restriction endonuclease digestion and analyzed by polyacrylamide gel electrophoresis, to distinguish alleles T and C of the IL1B gene, allowing for the determination of the genotypes and detection of the polymorphism. Results and Conclusions: The chronic periodontitis group displayed a higher percentage of the T allele (28%) when compared to the aggressive periodontitis group (10.7%, χ 2 = 5.24, p = 0.02, OR = 0.31, CI = 0.11–0.88) and to control group (8.7%, χ 2 = 7.11, p = 0.007, OR = 0.24, CI = 0.08–0.73). Our data suggested that the polymorphism in the locus +3954 of IL1B gene could be a risk factor for chronic periodontitis in a sample of Brazilian individuals.