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Behavior of human periodontal ligament cells on CO 2 laser irradiated dentinal root surfaces: an in vitro study
Author(s) -
Pant V.,
Dixit J.,
Agrawal A. K.,
Seth P. K.,
Pant A. B.
Publication year - 2004
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.2004.00751.x
Subject(s) - periodontal fiber , dentistry , in vitro , irradiation , dentinal tubule , materials science , chemistry , medicine , dentin , physics , biochemistry , nuclear physics
Objective: The aim of this study was to investigate the in vitro attachment behavior of human periodontal ligament fibroblasts on periodontally involved root surface after conditioning with CO 2 laser and to compare its efficacy with chemical conditioning agents, namely tetracycline hydrochloride, citric acid, hydrogen peroxide (H 2 O 2 ) and EDTA, using scanning electron microscopy. Methods: A total of 84 scaled and root‐planed specimens from periodontally involved single‐rooted human teeth showing hopeless prognosis were selected and assigned to two groups. One group was lased with a CO 2 laser (from 5 cm at 3 W for 0.8, 1.0 and 1.2 s), and the other group was treated with either tetracycline hydrochloride (2.5%), citric acid (saturated solution, pH 1), H 2 O 2 (6%) or EDTA (5%; pH 7.4) for 3 min. The specimens were then seeded with human periodontal ligament fibroblasts, incubated for either 12 h or 24 h, and then the cell attachment behavior was observed. Results: CO 2 laser irradiation for 1.0 s was found to be the most efficient, showing consistently good cell attachment with the highest mean value (15.00 ± 3.41 cells/10,000 µm 2 after incubation for 12 h and 29.17 ± 2.04 cells/10,000 µm 2 after 24 h), followed by irradiation for 0.8 s (13.11 ± 3.04 cells/10,000 µm 2 after incubation for 12 h and 22.91 ± 7.10 cells/10,000 µm 2 after 24 h). Charring was observed following irradiation for 1.2 s. Amongst chemical conditioning agents, citric acid was found to be the most efficient, with a mean cell attachment of 17.82 ± 2.16 cells/10,000 µm 2 after incubation for 12 h and 23.62 ± 1.94 cells/10,000 µm 2 after 24 h. EDTA and H 2 O 2 did not do well in the study. Conclusion: The results suggest that CO 2 laser irradiation for 1.0 s may promote comparatively better attachment of periodontal ligament fibroblast on dentinal root surfaces than the conventional chemical conditioning agents used in the study.