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Effect of heat treatment on bioactivities of enamel matrix derivatives in human periodontal ligament (HPDL) cells
Author(s) -
Nagano Takatoshi,
Iwata Takanori,
Ogata Yorimasa,
Tanabe Takako,
Gomi Kazuhiro,
Fukae Makoto,
Arai Takashi,
Oida Shinichiro
Publication year - 2004
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.2004.00733.x
Subject(s) - enamel matrix derivative , cementum , periodontal fiber , alkaline phosphatase , chemistry , matrix (chemical analysis) , in vitro , enamel paint , mtt assay , microbiology and biotechnology , dentistry , biochemistry , dentin , biology , medicine , chromatography , enzyme , regeneration (biology)
Objectives: It has been shown that Emdogain ® (EMD) containing enamel matrix derivative has cementum‐ and osteo‐promotive activities in vivo and in vitro . Nevertheless, the commercial sale of EMD was halted because it has some possible risk for infectiosity. At present, Emdogain ® Gel (Emd‐Gel) containing the EMD heated to avoid the infectiosity is commercially available. The purpose of this study was to compare the in vitro bioactivities of Emd‐Gel and EMD. Material and methods: Healthy human periodontal ligament (HPDL) cells were used to study the effect of Emd‐Gel and EMD on cell differentiation. The HPDL cells exposed to Emd‐Gel and EMD were evaluated for the following effects: (i) alkaline phosphatase (ALP) activity; (ii) mitogenic (MTT) assay; (iii) biomineralization activity; (iv) gene expressions using reverse transcription‐polymerase chain reaction (RT‐PCR). The effect of EMD with or without heat treatment was examined for ALP activity on the cell differentiation. Results: The effect of Emd‐Gel on ALP activity was greater than that of EMD. It was confirmed from the effect of EMD with heat treatment at 60°C, 80°C and 100°C on the ALP activity. The effect of Emd‐Gel on the biomineralization activity was also greater than that of the EMD. The Emd‐Gel has a stronger effect for the expression of osteoblast‐like phenotype than the EMD. Conclusion: The results indicate that the Emd‐Gel has greater bioactivities than the EMD in vitro .