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Effect of prostaglandin E 2 on recombinant human bone morphogenetic protein‐2‐stimulated osteoblastic differentiation in human periodontal ligament cells
Author(s) -
Taktguchi T.,
Kobayashi M.,
Nagashima C.,
Yamaguchi A.,
Nishihara T.,
Hasegawa K.
Publication year - 1999
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1999.tb02278.x
Subject(s) - chemistry , alkaline phosphatase , prostaglandin e2 , bone morphogenetic protein 2 , prostaglandin e , endocrinology , medicine , bone morphogenetic protein , endogeny , recombinant dna , periodontal fiber , osteoblast , in vitro , biochemistry , biology , enzyme , dentistry , gene
Recombinant human (rh) bone morphogenetic protein‐2 (BMP‐2) stimulates osteoblastic differentiation in cells isolated from human periodontal ligament (HPLC), and this action of rhBMP‐2 may be modulated by prostaglandins (PGs), which are local regulatory factors in the bone metabolism. In the present study, we investigated the effect of prostaglandin E 2 (PGE 2 ) on rhBMP‐2‐stimulated osteoblastic differentiation in cultured HPLC. rhBMP‐2 (500 ng/ml)‐stimulated alkaline phosphatase (ALPase) activity was enhanced by simultaneous treatment with low concentrations (10 −10 ‐10 −8 M) of PGE 2 , whereas a high concentration (10 −6 M) of PGE 2 suppressed it. rhBMP‐2 did not induce cyclo‐oxygenase‐2 (COX‐2) mRNA expression or subsequent PGE 2 production, whereas it remarkably suppressed rhIL‐1β‐induced COX‐2 mRNA expression and PGE 2 production. The rhBMP‐2 action on osteoblastic differentiation in HPLC was also enhanced by co‐treatment with 0.25 to 25 ng/ml of rh interleukin‐1β (IL‐1β). The ALPase activity stimulated by simultaneous treatment with rhBMP‐2 and rhIL‐1β was partially inhibited by addition of 10 −6 M of indomethacin, which completely inhibited rhIL‐lβ‐induced PGE 2 production. These results reveal that PGE 2 at different concentrations exerts a biphasic effect on BMP‐2‐stimulated osteoblastic differentiation in HPLC, BMP‐2 inhibits IL‐1β‐induced PGE 2 production through suppressing COX‐2 expression, and the BMP‐2‐stimulated osteoblastic differentiation may be enhanced by the endogenous PGE 2 induced by BMP‐2 and IL‐1 β. These suggest that BMP‐2 action on osteoblastic differentiation in HPLC may be modulated by PGE 2 in autocrine and paracrine fashions.