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Graft of autologous fibroblasts in gingival tissue in vivo after culture in vitro preliminary study on rats
Author(s) -
SimainSato F.,
Lahmouzi J.,
Heinen E.,
Defresne M. P.,
PauwGillet M. C.,
Grisar Th.,
Legros J. J.,
Legrand R.
Publication year - 1999
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1999.tb02260.x
Subject(s) - in vivo , trypan blue , tissue culture , connective tissue , in vitro , pathology , medicine , chemistry , biology , biochemistry , microbiology and biotechnology
Several grafting techniques and guided tissue regeneration techniques (GTR) have been well‐developed in periodontal surgery. However, these techniques could induce pain and side effects, such as a gingival recession during the healing period following the therapy. The graft of a small autologous connective tissue, using non‐invasive surgical techniques could yield several benefits for the patients. Our preliminary study explores the feasibility of collecting healthy gingival tissues, culturing them in vitro to amplify rat gingival fibroblasts (RGF) and inoculating the obtained cells into autologous rat gingival tissues in vivo. Gingival tissues samples were cultured as explains as described by Freshney et al. and Adolphe. Confluent cells surrounding explants were detached after 7 d of culture from Petri dishes using 0.05% trypsin and designated “first transferred cells” (T1). At the third passage (T3), cells cultured as monolayer were either examined under microscopy ‐ phase contrast, scanning, or transmission electron ‐ or numerated after trypan blue exclusion test. Autologous RGF labelled with fluorochrome were inoculated at the vestibular and palatine site of gingival tissue close to the superior incisors. In this preliminary study, 12 Wistar rats were used; for each, 2 biopsies were dissected and fixed for phase contrast or fluorescence microscopy. On d 1, 3 and 7 after injection in rat gingival tissues, fluorochrome‐labelled cells could be detected in all these.