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Lipopolysaccharide from Actinobacillus actinomycetemcomitans stimulates production of interleukin‐1 β, tumor necrosis factor‐α, interleukin‐6 and interleukin‐1 receptor antagonist in human whole blood
Author(s) -
Blix I. J. Schytte,
Helgeland K.,
Hvattum E.,
Lyberg T.
Publication year - 1999
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1999.tb02219.x
Subject(s) - proinflammatory cytokine , lipopolysaccharide , interleukin 1 receptor antagonist , interleukin , tumor necrosis factor alpha , actinobacillus , receptor antagonist , cytokine , microbiology and biotechnology , chemistry , interleukin 1 receptor , ex vivo , immunology , biology , receptor , periodontitis , inflammation , medicine , antagonist , in vitro , biochemistry
Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) is supposed to be an important etiological agent in localized juvenile periodontitis (LJP). We have studied the effect of lipopolysaccharide (LPS) extracted from these periodontopathogenic bacteria on synthesis of the proinflammatory cytokities, interleukin‐1β (IL‐1β), tumor necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6) and the anti‐inflammatory cytokine IL‐1 receptor antagonist (IL‐lra) in human whole blood. LPS from A. actinomycetemcomitans in concentrations ≥ 1 ng/ml induced a significant production of all these proinflammatory cytokines, whereas LPS from Escherichia coli (E. coli) , strain 026:B6 had to be added in concentrations ≥1 μg/ml to obtain a similar effect. Similarly, LPS from A. actinomycetemcomitans ≥0.1 ng/ml resulted in production of IL‐1ra, while LPS from E. coli 026:B6 had to be added at ≥10 ng/ml to obtain similar effects. It has been suggested that the ratio between production of proinflammatory and anti‐inflammatory cytokines may influence the outcome of periodontal diseases. Other in vitro and in vivo studies have, however, indicated that very large excesses (100‐1000 times) of IL‐1ra compared to IL‐1β are required to shift the IL‐1ra:IL‐1β ratio in favor of an inhibition of IL‐1 bioactivity. In our ex vivo system, we found that stimulation with extremely low doses of A. actinomycetemcomitans LPS (0.1‐1 ng/ml) resulted in IL‐1ra production solely, without concomitant production of IL‐1β, the excess of IL‐1ra over IL‐1β peaking at 1 ng/ml, which accordingly should suggest that LPS from A. actinomycetemcomitans primarily has proinflammatory effects.