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The influence of prostaglandins on steroid conversions by human gingival fibroblasts
Author(s) -
Soory M.,
Gower D. B.
Publication year - 1998
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1998.tb02342.x
Subject(s) - dihydrotestosterone , endocrinology , medicine , chemistry , testosterone (patch) , metabolism , stimulation , androgen , hormone , biology
The aim of this investigation was to study the effects of prostaglandins E 1 and E 2 (PGE 1 and PGE 2 ) on the accumulation, release and metabolism of C 19 steroids by human gingival fibroblasts (HGF) and gingivae, due to their anabolic potential in inflammatory repair. For the accumulation studies, HGF were incubated with 14C‐testosterone at timed intervals and the cell‐digests analysed for label uptake. The release of 5α‐dihydrotestosterone (DHT) by HGF was studied by preincubating the cells with 14C‐DHT and reincubating with cold steroid to quantify its release at timed intervals. For the metabolic studies, HGF/gingival tissue were incubated with 14C‐testosterone and serial concentrations of PGE 1 and PGE 2 to study their effects on the synthesis of DHT. The incubations were terminated at 24 h and extracted metabolites were analysed and quantified. The accumulation of 14C‐testosterone by human gingival fibroblasts was elevated 3‐fold at 24 h by PGE 1 (n = 3, p < 0.001; 1‐way ANOVA). The release of 14C‐DHT was enhanced nearly 2‐fold by PGE X (n = 3, p < 0.001), compared with controls. Both PGE 1 and PGE 2 caused 2‐fold increases in DHT synthesis by HGF and 3‐fold increases in 4‐androstenedione formation ( n =4, p < 0.001). With the tissue incubations PGE 1 /PGE 2 caused 3‐4‐fold increases in DHT synthesis ( n = 5, p<0.005; Wilcoxon signed rank statistic for paired observations). Direct stimulation of the accumulation/release and metabolism of these steroids by prostaglandins in gingivae may contribute to the anabolic potential of androgens in inflammatory periodontal disease.