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Biofilm formation by Porphyromonas gingivalis and Streptococcus gordonii
Author(s) -
Cook G. S.,
Costerton J. W.,
Lamont R. J.
Publication year - 1998
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1998.tb02326.x
Subject(s) - streptococcus gordonii , biofilm , porphyromonas gingivalis , microbiology and biotechnology , saliva , colonization , chemistry , streptococcus sanguinis , bacteria , streptococcus mutans , pathogen , biology , biochemistry , genetics
Confocal scanning laser microscopy (CSLM) was used to visualize and quantify biofilm formation by the oral bacteria Streptococcus gordonii and Porphyromonas gingivalis. A saliva‐coated glass coverslip under continuous bacterial challenge and conditions of low shear force was used to investigate attachment to the salivary pellicle and also the effect of cell‐cell interactions on the extent of colonization and biofilm development. S. gordonii bound to the salivary pellicle and outcompeted P. gingivalis for attachment sites. Both P. gingivalis and S. gordonii failed to establish substantial biofilm formation independently. However, biofilm formation did occur subsequent to initial adherence of P. gingivalis to S. gordonii cells deposited on the salivary pellicle. The commensal species S. gordonii may, therefore, provide an attachment substrate for colonization and biofilm accretion by the potential pathogen, P. gingivalis.