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Characterization of fibromodulin isolated from bovine periodontal ligament
Author(s) -
Watanabe Toru,
Kubota Takao
Publication year - 1998
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1998.tb02285.x
Subject(s) - keratan sulfate , chemistry , biochemistry , proteoglycan , polyclonal antibodies , microbiology and biotechnology , antibody , extracellular matrix , biology , immunology
Although several proteoglycans (PGs) have been reported in bovine periodontal ligament (PDL), the composition of PGs in PDL has been poorly characterized. In the present study, we isolated and characterized keratan sulfate‐substituted PG (fibromodulin) in bovine PDL. Fibromodulin was purified from 4 m guanidine hydrochloride (GdmCl) extracts of bovine PDL tissues using DEAE Sephacel ion‐exchange chromatography and preparative electrophoresis. Fibromodulin appeared as a single polydisperse band with an apparent molecular weight (MW) of 80, 000 (80 kDa) on SDS‐PAGE. Digestion of fibromodulin with keratanase or neuraminidase reduced the apparent molecular size, and N‐glycanase treatment produced core protein bands of around 40 kDa. Fibromodulin reacted with keratan sulfate monoclonal antibody (5D4) and fibromodulin polyclonal antibodies (α‐FM). The keratanase‐digested fibromodulin reacted with α‐FM, but not with 5D4. These data suggest that fibromodulin is one of the small PGs in the PDL‐matrix and may fulfill construction and maintenance functions in this tissue.