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Distribution of macrophage lineage cells in rat gingival tissue after topical application of lipopolysaccharide: an immunohistochemical study using monoclonal antibodies: OX6, ED1 and ED2
Author(s) -
Miyauchi Mutsumi,
Takata Takashi,
Ito Hiroshi,
Ogawa Ikuko,
Kudo Yasusel,
Takekoshi Toshitsugu,
Nikai Hiromasa
Publication year - 1998
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1998.tb02209.x
Subject(s) - immunohistochemistry , monoclonal antibody , macrophage , pathology , lipopolysaccharide , antibody , medicine , distribution (mathematics) , immunology , biology , biochemistry , in vitro , mathematical analysis , mathematics
To discuss the role of macrophage lineage cells on the periodontal tissue destruction, we immunohistochemically examined the phenotype and the dynamics of macrophage lineage cells 1 or 3 h or 1, 2, 3 or 7 d after topical application of LPS (5 mg/ml in physiological saline) from the rat gingival sulcus using 3 monoclonal antibodies: OX6 (antigen‐presenting cells), ED1 (monocytes, macrophages and dendritic cells) and ED2 (resident macrophages). We could detect at least 3 different types of macrophage lineage cells, namely OX6 + /ED1 + /ED2 − dendritic cells and exudate macrophages and ED2 + resident macrophages. After LPS application the majority of macrophage lineage cells accumulated in the subjunctional epithelial area were newly extravasated OX6 + /ED1 + /ED2 − dendritic cells or macrophages. The number Department of Oral Pathology, Hiroshima of these cells increased progressively with time and reached a maximum level at University School of Dentistry, d 2. On the other hand, number and tissue distribution of ED2 + resident macrophages did not change. These results indicate that several types of macrophage lineage cells exist in rat gingival tissue and suggest that dendritic cells and exudate macrophages transiently accumulated after LPS application are responsible for various host immune response and tissue destruction caused by LPS.