Premium
Transforming growth factor‐β response and expression in junctional and oral gingival epithelial cells
Author(s) -
Lu H.,
Mackenzie I. C.,
Levine A. E.
Publication year - 1997
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1997.tb00579.x
Subject(s) - transforming growth factor , biology , extracellular matrix , receptor , microbiology and biotechnology , biochemistry
The junctional (JE) and oral gingival (OGE) epithelium show distinct morphological phenotypes and express different cell surface and keratin markers. Transforming growth factor‐β (TGF‐β) has been shown to stimulate extracellular matrix formation and inhibit proteolytic matrix degradation in periodontal wound healing. To elucidate potential roles of TGF‐β in gingival epithelial regeneration and reattachement, the present study examined the effects of TGF‐β on JE and OGE cell growth and determined the patterns of expression of mRNAs for the TGF‐β isotypes β1, β2 and β3 and TGF‐β receptor types I, II and III. Primary cell cultures were initiated from JE and OGE and the cell phenotypes confirmed using monoclonal antibodies to specific keratins. TGF‐β induced a significant growth inhibition in OGE cells derived from 6 different patients with a mean inhibition of 46% and a range of 16–70% (p=0.031). Although responses varied between patients, in general maximum inhibition occurred at 10 ng/ml TGF‐β. JE cells from 5 patients showed no significant growth inhibition by TGF‐β (p=0.125). Greater expression of TGF‐β2 and receptor type I mRNA was found in OGE than JE cells and thus appeared to be associated with differentiating epithelial cells. JE cells expressed more TGF‐β type II receptor specific mRNA than did OGE cells, but TGF‐β1 mRNA expression was similar in JE and OGE cells. JE or OGE cultures derived from 2 of 3 patients showed expression of mRNA for the TGF‐β type III receptor. TGF‐β3 mRNA was not detected in any of the JE or OGE samples examined. The greater sensitivity of OGE than JE to the growth inhibiting effects of TGF‐β correlated with higher expression of receptor type I mRNA which, together with the type II receptor, is required for sensitivity to growth inhibition by TGF‐β. The results suggest that, in addition to structural differences, the development of functional differences in the responses of JE and OGE to TGF‐β may be associated with the formation of JE from OGE cells and the reformation of attachement after periodontal surgery.