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In situ hybridization study on tissue inhibitors of metalloproteinases (TIMPs) mRNA‐expressing cells in human inflamed gingival tissue
Author(s) -
Kubota Takehiko,
Matsuki Yutaka,
Nomura Takashi,
Hara Kohji
Publication year - 1997
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1997.tb00559.x
Subject(s) - in situ hybridization , connective tissue , matrix metalloproteinase , epithelium , gingivitis , periodontitis , pathology , junctional epithelium , messenger rna , biology , chemistry , medicine , dentistry , gene , biochemistry
This study presents the exact cell types and localization of tissue inhibitors of metalloproteinases (TIMPs) production sites in periodontal diseased gingiva by means of in situ hybridization. Gingival tissue specimens were fixed, embedded and hybridized in situ with specific digoxigenin‐labeled cRNA probes (386 and 496 bp). TIMP‐1 and ‐2 mRNAs were expressed on macrophages, mononuclear cells, capillary endothelial cells and some fibroblasts throughout the gingival tissue. In periodontitis, TIMP‐1 and ‐2 mRNA‐expressing cells showed significantly different localization. TIMP‐1 mRNA was broadly observed in the gingival connective tissue while TIMP‐2 mRNA was predominantly expressed in the connective tissue adjacent to the pocket epithelium (p < 0.01). Fewer TIMPs mRNA were observed in minimal gingivitis than in periodontitis, especially in the middle zone of gingival tissue. Thus, TIMP‐1 and TIMP‐2 mRNA was detected differentially and site‐specifically in periodontal diseased gingival tissue.