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The synthesis of 5α ‐ dihydrotestosterone from androgens by human gingival tissues and fibroblasts in culture in response to TGIF‐β and PDGF
Author(s) -
Kasasa S. C.,
Soory M.
Publication year - 1996
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1996.tb00498.x
Subject(s) - androstenedione , medicine , endocrinology , platelet derived growth factor receptor , testosterone (patch) , dihydrotestosterone , chemistry , androgen , cell culture , platelet derived growth factor , growth factor , biology , receptor , hormone , genetics
The effects of TGF‐β and PDGF on the metabolic conversion of 14C‐testosterone by human gingival tissue (HGT) from 5 subjects was investigated. The metabolic conversions in response to TGF‐β and PDGF were also studied in 4‐6 cell‐lines of cultured gingival fibroblasts, using 14C‐testosterone and 14C‐4‐androstenedi one as substrates. Duplicate incubations of HGT were performed in Eagle's MEM + 10% FCS and optimal stimulatory concentrations of TGF‐β/PDGF for 24 h. Similar incubations were performed in duplicate with cell‐lines of cultured gingival fibroblasts, TGF‐β PDGF, 14C‐testosterone/14C‐4‐androstenedione in Eagle's MEM + 10% FCS. The radioactive metabolites were extracted, separated and quantified. With HGT, TGF‐β and PDGF caused 2.5/12‐fold increases in DHT synthesis (p< 0.1; Wilcoxon signed rank test) and 3.4/12‐fold increases in 4‐androstenedione formation (p<0.1) from 14C‐testosterone. PDGF increased DHT and testosterone synthesis from 14C‐4‐androstenedione by 3‐fold in gingivae (p<0.1). With cell‐lines, average values of duplicate incubations showed 2.8/2‐fold increases in DHT synthesis from 14C‐testosterone in response to TGF‐β/PDGF (p<0.1; p<0.2) and 2.4/2‐fold increases in 4‐androstenedione synthesis (p<0.1; p<0.2). With 14C‐4‐androstenedione as substrate, TGF‐β/PDGF caused 1.611.9fold increases in DHT synthesis compared with controls (p<0.05; p<0.1) and 1.711.5‐fold increases in testosterone formation from this substrate (p<0.05; p<0.1). Due to the strong implications of TGF‐β/PDGF and anabolic androgens on matrix repair, significant increases in DHT synthesis from 2 androgenic substrates in response to TGF‐β and PDGF are of particular relevance to inflammatory repair processes.