Premium
Comparative effects of plateletderived growth factor‐BB and insulin‐like growth factor‐I, individually and in combination, on periodontal regeneration in Macaca fascicularis
Author(s) -
Giannobile William V.,
Hernandez Rafaél A,
Finkelman Richard D.,
Ryarr Susan,
Kiritsy Christopher P.,
D'Andrea Mark,
Lynch Samuel E.
Publication year - 1996
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1996.tb00497.x
Subject(s) - growth factor , regeneration (biology) , insulin like growth factor , platelet derived growth factor , platelet derived growth factor receptor , periodontitis , ligature , medicine , endocrinology , biology , receptor , microbiology and biotechnology
Platelet‐derived growth factor (PDGF) and insulin‐like growth factor I (IGF‐I) in combination have previously been shown to enhance periodontal regeneration. The objective of this study was to further characterize the biological effects of this combination of growth factors in non‐human primates and compare the effects to those of each growth factor individually. Ligature‐induced periodontitis was initiated in 10 cynomolgus monkeys. After periodontal lesions were established, surgery was performed, and either a methylcellulose gel vehicle or vehicle containing 10 μg each of either PDGF‐BB, IGF‐I or both PDGF‐BB and IGF‐I was applied to exposed root surfaces. Biopsies were taken 4 and 12 wk after treatment and the extent of periodontal regeneration was assessed by histomorphometry. At both 4 and 12 wk vehicle‐treated lesions generally revealed minimal osseous defect fill (ODF) (8.5±2.1% and 14.5±5.7%, respectively) and new attachment (NA) (34.1±5.2% and 26.6±10.5%, respectively). IGF‐I treatment did not significantly alter healing compared to vehicle in any parameter at both 4 and 12 wk. PDGFBB‐treated sites exhibited significant (p<0.05) regeneration of NA (69.6±12.0%) at 12 wk; trends for PDGF‐BB treatment effect were also observed in other parameters at 4 and 12 wk. although these increases were not statistically significant. Treatment with PDGF‐BB/IGF‐I resulted in 21.6±5.1 % and 42.5±8.3% ODF at 4 and 12 wk, respectively, and 64.1±7.7% and 74.6±7.4% NA at 4 and 12 wk, respectively (all significantly greater than vehicle, p<0.05). The results from this study demonstrated that: 1) IGF‐1 alone at the dose tested did not significantly alter periodontal wound healing; 2) PDGF‐BB alone significantly stimulated NA, with trends of effect on other parameters; and 3) the PDGF‐BB/IGF‐I combination resulted in significant increases in NA and ODF above vehicle at both 4 and 12 wk.