Premium
Influence of sE‐selectin and L‐selectin on the regulation of cell migration during chronic periodontitis
Author(s) -
Krugluger W.,
Nell A.,
Solar P.,
Matejka M.,
BoltzNitulescu G.
Publication year - 1995
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1995.tb01274.x
Subject(s) - l selectin , e selectin , chemistry , flow cytometry , cell adhesion molecule , immunofluorescence , sialyl lewis x , selectin , microbiology and biotechnology , periodontitis , andrology , medicine , cell adhesion , immunology , endocrinology , cell , biochemistry , biology , antibody
Summary Soluble endothelial leukocyte adhesion molecule‐1 (sE‐selectin) levels in peripheral blood (PB) and gingival capillary blood (GCB) of both healthy donors (HD) and patients with adult periodontitis (AP) were assayed by ELISA. Binding of sE‐selectin to polymorphonuclear neutrophils (PMN) from PB, GCB and crevicular fluid (GF), and expression of L‐selectin and sialyl‐Lewis x (sLe x ) on these cells were analyzed by immunofluorescence and flow cytometry. No significantly enhanced serum levels of sE‐selectin in patients with AP. compared to HD (28±5 ng/ml vs 19±3 ng/ml, respectively), and no differences in the concentration of sE‐selectin in GCB (16±1 ng/ml vs 16±2 ng/ml, respectively) were observed. On PB‐PMN no significant differences in the expression of L‐selectin and sLe x were found and binding of sE‐selectin to PB‐PMN was comparable between HD and patients with AP. Binding of sE‐selectin to GCB‐PMN was significantly higher in patients with AP compared to HD (mean channel fluorescence (MCF)=88.5± 13.2 vs MCF=24.2±5.3, respectively). The expression of sLe x on GCB‐PMN did not differ significantly between the two groups. A significant decrease in the expression of the adhesion molecule L‐selectin on GCB‐PMNs compared to PB‐PMN was found in patients with AP but not in HD. CF‐PMN showed decreased expression of both L‐selectin and sLe x compared to PMN from PB and GCB, both in HD and patients with AP. Taken together, these data suggest that PMN from patients with AP had reduced selectin‐mediated adhesive capabilities to inflamed gingival endothelium.