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Polyamines found in gingival fluid enhance the secretory and oxidative function of human polymorphonuclear leukocytes in vitro
Author(s) -
Walters J.D.,
Chapman K.J.
Publication year - 1995
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1995.tb01269.x
Subject(s) - spermidine , spermine , superoxide , in vitro , granule (geology) , putrescine , chemistry , reactive oxygen species , lysis , oxidative phosphorylation , granulocyte , polymorphonuclear leukocyte , microbiology and biotechnology , biochemistry , immunology , biology , enzyme , paleontology
Summary Many bacterial and host cells contain large amounts of polyamines that can be released at infection sites as a result of cell lysis. Consequently, the putrescine and spermidine content of gingival fluid from inflamed periodontal pockets (0.1 to 1 mM) is sharply elevated in comparison to peripheral blood. At these levels, polyamines potentiated fMet‐Leu‐Phe‐induced Ca 2+ signaling in polymorphonuclear leukocytes (PMNs) in vitro . Consistent with the essential role of Ca 2+ signaling in PMN activation, secondary granule release and superoxide anion production by fMet‐Leu‐Phe‐stimulated PMNs was enhanced in the presence of polyamines. Thus, polyamines may play a local role in modulating the antimicrobial activity of PMNs in periodontal disease.