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Cytotoxic effect of vesicles produced by Porphyromonas gingivalis on fibroblasts in culture
Author(s) -
Gulgand M.,
Pallen P.,
Mouton C.,
BonnaureMallet M.
Publication year - 1995
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1995.tb01263.x
Subject(s) - porphyromonas gingivalis , sulforhodamine b , cytotoxic t cell , vesicle , flow cytometry , cytotoxicity , chemistry , microbiology and biotechnology , biology , in vitro , bacteria , biochemistry , genetics , membrane
It has been shown that the vesicles produced by Porphyromonas gingivalis under certain growth conditions contribute to its pathogenicity. In this study, we demonstrate the cytotoxic effect of the vesicles using two methods: on quantitative (the colorimetric cytotoxicity test using sulforhodamine B) and the other qualitative (flow cytometry).