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Elastin derived peptides protect elastic fibres degradation by human neutrophil elastase: in vitro and in vivo studies using a mechanically induced rat gingival inflammatory model
Author(s) -
BonnaureMallet M.,
Godeau G.,
Tixier J. M.,
LebonPelletier P.,
Chambon Y.,
Robert L.,
Homebeck W.
Publication year - 1995
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1995.tb01253.x
Subject(s) - elastin , elastase , in vivo , chemistry , sephadex , in vitro , collagenase , pancreatic elastase , peptide , infiltration (hvac) , penetration (warfare) , periodontitis , biochemistry , pathology , enzyme , medicine , materials science , biology , composite material , microbiology and biotechnology , operations research , engineering
An elastin peptide (kE 57 ) obtained from organoalkaline hydrolysis of calf ligamentum nuchae insoluble elastin, was isolated by gel permeation on Sephadex G150 and high performance liquid chromatography on a TSK G 3000 SW column. It possessed an average Mr=57,000 and similar amino acids composition as its insoluble counterpart. KE 57 behave as a competitive inhibitor of human neutrophil elastase (HNE) with K I =1.4 μM; it also inhibited porcine pancreatic elastase (PPE) but less efficiently K I =180 μM. Identification of elastic fibres in rat gingiva was ascertained by light and electron microscopic studies. Morphometric studies indicated that rat gingiva contained similar levels of elastic fibres (=2%) as human skin; elastic fibres networks from both tissues also displayed high structural analogy. Gingival chronic inflammation was induced in rats by mechanical impaction associated with an hyperglucidic diet. After 5 weeks, the levels of rat gingiva elastic fibres, decreased from Vv= 1.94±0.1% to Vv= 1.02±0.06%. Local injections of kE 57 : 100 μig per day, 5 days a week for 5 weeks did restore the integrity of the gingiva elastic fibres network: Vv= 1.84±0.1. Without influencing leucocyte infiltration, it is proposed that elastin‐derived peptides, acting as potent competitive inhibitor of neutrophil elastase involved in periodontitis, might be of therapeutic value.