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Scanning electron microscopy of Eikenella corrodens colony morphology variants
Author(s) -
Cobb C. M.,
Helber J. T.,
Hirschberg R.
Publication year - 1994
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1994.tb01242.x
Subject(s) - eikenella corrodens , biology , morphology (biology) , scanning electron microscope , colony morphology , strain (injury) , microbiology and biotechnology , bacteria , anatomy , zoology , genetics , materials science , composite material
Eikenella corrodens is a gram‐negative, human pathogen which exhibits colony morphology variation. Scanning electron microscopy (SEM) was used to examine large (non‐corroding) and small (corroding) colony variants from the type strain (ATCC 23834) and two clinical isolates (strains VA1 and CM1), Large colonies were large, flatter, and appeared relatively featureless compared to small colonies and had even, smooth colony margins. Small colonies were more raised from the medium surface, and often had a central raised region surrounded by flatter border. Cells on the surface of large colonies were more regularly arranged at the colony edge, and end‐to‐end rows of cells around the colony were seen in some strains. Cells in the center of the upper surface of small colonies were usually randomly arranged. Within cross sections of small colonies, cells were arranged randomly or perpendicular to the medium; in large colonies, cells were random or arranged horizontally. Amorphous, “slime” material was often seen covering groups of cells in large and small variants. An unusual variant, possibly a mutant, which combined features of both colony types was isolated.