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Effect of long‐term methotrexate‐induced neutropenia on experimental periodontal lesion in rats
Author(s) -
Yoshinari N.,
Kameyama Y,
Aoyama Y.,
Nishiyama H.,
Noguchi T.
Publication year - 1994
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1994.tb01240.x
Subject(s) - medicine , dental alveolus , lesion , saline , methotrexate , resorption , bone resorption , dentistry , neutropenia , molar , surgery , chemotherapy
The effects of long‐term methotrexate (MTX)‐induced neutropenia on the periodontal lesion in rats were investigated histologically, histometrically and bacteriologically. A nylon thread was inserted into the interdental gingiva between the 1st and 2nd right maxillary molars of the animals 3 weeks before an application of MTX. The animals were then divided into Groups A and B. Group B were injected intraperitoneally with 1.0 mg/kg of MTX 3 times per week for 9 weeks. Group A received saline as a control. Five animals were killed at the 1st, 3rd, 5th, 7th, and 9th week. In Group A, the neutrophils did not decrease during these 9 weeks. In Group B, however, the neutrophils decreased during the 3rd to 9th week. Whereas the experimental side of Group A showed only moderate alveolar bone resorption between the 7th and 9th week, [in Group B] a marked alveolar bone resorption occurred in Group B occurred in the same period. Alveolar bone loss in the experimental side of Group B was significantly greater (p < 0.01) than in Group A in the 7th to 9th weeks. The percentage of gram‐negative rods increased in both control and experimental sides of Group B at the 9th week. The results of the present study indicate that neutropenia is induced by a long‐term application of MTX in rats and that alveolar bone destruction increases as time goes by in the area where a nylon thread was inserted.