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Increased respiratory burst activity is associated with normal expression of IgG‐Fc‐receptors and complement receptors in peripheral neutrophils from patients with juvenile periodontitis
Author(s) -
Leino L.,
Hurttia H. M.,
Sorvajärvi K.,
Sewon L. A.
Publication year - 1994
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1994.tb01211.x
Subject(s) - zymosan , respiratory burst , opsonin , receptor , endocrinology , medicine , complement receptor , n formylmethionine leucyl phenylalanine , immunology , phagocytosis , biology , fc receptor , periodontitis , chemistry , chemotaxis , antibody , complement system , biochemistry , in vitro
The respiratory burst activity in peripheral neutrophils from nine patients with localized juvenile periodontitis (LJP) and age‐ and sex‐matched healthy controls was studied by measuring the intensity of luminol‐enhanced chemiluminescence (CL) induced by unopsonized and three differently opsonized zymosan particles, formyl‐methionyl‐leucyl‐phenylalanine (FMLP) or phorbol myristate acetate (PMA). The neutrophils from LJP patients showed in general more intense CL with all activators than did their controls. Particulary, the CL response induced by unopsonized zymosan particles and FMLP were significantly higher (p < 0.05 and 0.001). When comparisons were made between female LJP patients (n = 6) and matched controls, also serum‐opsonized and IgG‐opsonized zymosan particles produced CL was significantly increased (p < 0.05). In order to determine whether the elevated CL responses to zymosan particles were due to altered levels of the interacting receptors on neutrophil surface, an immunofluorescence analysis of the expression of IgG‐Fc‐receptors (FcR) and complement receptors (CR) was performed with flow cytometry. No significant difference in the expression of FcRII, FcRIII, CRl and CR3 was detected in LJP group compared to controls. Since the elevated CL responses can not be explained by changes in receptor numbers it is hypothesized that the increased respiratory burst activity in LJP may be caused by altered post receptor signalling pathway.