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Expression of TIMP‐1, TIMP‐2 and collagenase mRNA in periodontitis‐affected human gingival tissue
Author(s) -
Nomura Takashi,
Takahashi Tokuya,
Hara Kohji
Publication year - 1993
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1993.tb01079.x
Subject(s) - collagenase , periodontitis , gene expression , interstitial collagenase , matrix metalloproteinase , messenger rna , gene , reverse transcription polymerase chain reaction , tissue inhibitor of metalloproteinase , in vivo , reverse transcriptase , real time polymerase chain reaction , biology , microbiology and biotechnology , chemistry , polymerase chain reaction , andrology , medicine , enzyme , genetics , biochemistry
Collagenolysis in periodontitis is thought to be modulated by the expression of three genes, collagenase, tissue inhibitors of metalloproteinases‐1 and ‐2 (TIMP‐1 and ‐2). We assessed the possible difference in TIMP‐1, TIMP‐2 and collagenase mRNA levels between gingival samples from patients with periodontitis and those from healthy subjects by reverse transcription‐polymerase chain reaction (RT‐PCR). This technique allows detection of transcripts from a very small sample quantity. The experiments showed that levels of TIMP‐1 and collagenase transcripts relative to β‐action are significantly higher in the diseased group than in healthy controls (8.11±0.83 versus 1.38±0.28% for TIMP‐1 and 0.50±0.10 versus 0.0075±0.0024% for collagenase, respectively). The difference in TIMP‐2 between the two groups (2.91±0.46 versus 1.84±0.87%) did not differ. Therefore, the host would have responded to the increase in collagenase level by preferentially producing TIMP‐1 against tissue destruction. The differential gene expression of TIMP‐1 and TIMP‐2 in our study may account for a distinct genetic regulation of TIMP‐1 and ‐2 in vivo .