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Efficient and unbiased estimation of volume and area of tissue components and cell number in gingival biopsies
Author(s) -
Verwer R. W. H.,
RaberDurlacher J. E.
Publication year - 1993
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1993.tb01075.x
Subject(s) - stereology , sampling (signal processing) , estimation , dentistry , volume (thermodynamics) , pathology , computer science , medicine , computer vision , management , filter (signal processing) , economics , physics , quantum mechanics
Recently, the methodology for volume and cell number estimation from sectioned tissue has undergone rapid progress. To data there are no indications in the literature that investigators in periodontal research are aware of this progress, published mainly in the disciplines of stereology and morphometry. This is unfortunate, as these developments could be profitably exploited in periodontal pathology. In this review we discuss the dangers of some currently used quantitative methods and indicate how simple unbiased methods together with an appropriate systematic sampling scheme lead to an efficient estimation of cell number and volume of tissue or tissue components.