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Micropuncture measurements of interstitial fluid pressure in normal and inflamed gingiva in rats
Author(s) -
Fjærtoft M.,
Johannessen A. C.,
Heyeraas K. J.
Publication year - 1992
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1992.tb01828.x
Subject(s) - ligature , connective tissue , molar , pathology , interstitial fluid , epithelium , chemistry , inflammation , anatomy , medicine , dentistry
Interstitial fluid pressure (IFF) in normal and inflamed gingiva of rats was measured by sharpened glass capillaries (diameter 2–4 μ) connected to a servocontrolled counterpressure system ad modum Wiederhielm. Gingival inflammation was induced by a steel ligature placed unilaterally around the first maxillary molar. After 8 weeks, the ligature was removed and measurements of IFF in normal and experimentally inflamed gingiva were made by puncture through the intact oral epithelium. Average IFF on the control side was 3.5 mm Hg in free, and 7.4 mm Hg in attached gingiva. Compared to the control side. IFF on the experimental inflamed side was elevated significantly (p<0.05) both in free and attached gingiva. In the ligated inflamed side IFF in free gingiva averaged 6.7 mm Hg, whereas in attached gingiva it showed a mean value of 10.4 mm Hg. Histological examinations showed a dense infiltrate of inflammatory cells in the connective tissue, while the contralateral control side showed normal features of epithelial and connective tissue. The present finding of increased IFF in the inflamed gingiva may in theory contribute to augmented crevicular fluid flow during inflammation.