Premium
A sensitive enzymatic method (SK‐013) for detection and quantification of specific periodontopathogens
Author(s) -
Ishihara Kazuyuki,
Naito Yuko,
Kato Tetsuo,
Takazoe Ichiro,
Okuda Katsuji,
Eguchi Toru,
Nakashima Koichi,
Matsuda Naoki,
Yamasaki Kyoko,
Hasegawa Kenji,
Suido Hirohisa,
Sugihara Kunio
Publication year - 1992
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1992.tb01807.x
Subject(s) - treponema denticola , porphyromonas gingivalis , microbiology and biotechnology , prevotella intermedia , bacteroides , bacteria , bacteroidaceae , periodontitis , gingival and periodontal pocket , chemistry , dental plaque , enzyme , anaerobic bacteria , periodontal disease , biology , medicine , dentistry , biochemistry , genetics
Porphyromonas gingivalis, Bacteroides forsythus , and Treponema denticola have been found to predominate in periodontal pockets of patients with adult periodontitis. These microorganisms hydrolyze the synthetic peptide N‐benzoyl‐DL‐arginine‐2‐naphthylamide (BANA). In this study, we developed an enzymatic method, designated SK‐013, to detect the existence of these microorganisms in subgingival plaque bacteria. This enzymatic method was based on the observation of the hydrolysis of N‐carbobenzoxy‐glycyl‐glycyl‐arginyl‐3,5‐dibromo‐4‐hyd‐roxyaniline (N‐CBz‐Gly‐Gly‐Arg‐DBHA) and made more sensitive by adding an enhancing system. The SK‐013 was specifically positive for P. gingivalis, B. forsythus, T. denticola , and some strains of Capnocytophaga species, but was not specific for any of the other bacterial strains tested. This SK‐013 system may be valuable for detection and quantification of periodontal disease‐associated bacteria in subgingival plaque and thus for diagnosis of periodontal infections.