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The mechanisms of Eikenella corrodens aggregation by salivary glycoprotein and the effect of the glycoprotein on oral bacterial aggregation
Author(s) -
Ebisu Shigeyuki,
Nakae Hideaki,
Fukuhara Hironobu,
Okada Hiroshi
Publication year - 1992
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1992.tb01745.x
Subject(s) - eikenella corrodens , microbiology and biotechnology , actinobacillus , bacteria , bacteroides , porphyromonas gingivalis , chemistry , galactose , dental plaque , biology , biochemistry , genetics
The mechanism of aggregation of Eikenella corrodens 1073 with E. corrodens aggregating factor (EcAF) which was purified from submandibular‐sublingual (SM‐SL) saliva was investigated. Heating (100°C, 10 minutes) or protease treatment of E. corrodens cells abolished the aggregating activity. The aggregation was inhibited by adding N‐acetyl‐D‐galactosamìne (GalNAc) and saccharides which contain a galactose residue at the non‐reducing end. The aggregating activity was sensitive to EDTA and was restored by Ca 2+ but not by Mn 2+ or Mg 2+ . Neuraminidase treatment of EcAF increased their ability to aggregate. E. corrodens , suggesting that the sialic acids on EcAF interfere with aggregation. These data suggest that the aggregation of E. corrodens 1073 with EcAF is mediated by specific interactions between a bacterial cell surface lectin‐like substance and a complementary GalNAc‐like receptor. EcAF also aggregated 16 strains of oral bacteria including periodontopathic bacteria such as Porphyromonas (Bacteroides) gingivalis 381 and Actinobacillus actinomycetemcomitans ATCC29522; however, those aggregations were not inhibited by GalNAc. Therefore, EcAF appears to have more than two types of bacterial binding site and plays important roles in accumulation of dental plaque by forming a complex network of plaque bacteria including periodontopathic strains.