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Effect of interleukin‐1 β on gene expressions and functions of fibroblastic cells derived from human periodontal ligament
Author(s) -
Takeshita Akira,
Niu Zhon Gying,
Hanazawa Shigemasa,
Takara Iwao,
Higuchi Hiroaki,
Katayama Ikuemon,
Kitano Shigeo
Publication year - 1992
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1992.tb01675.x
Subject(s) - periodontal fiber , biology , cytokine , interleukin , alkaline phosphatase , microbiology and biotechnology , messenger rna , interleukin 6 , tumor necrosis factor alpha , gene expression , endocrinology , medicine , immunology , gene , enzyme , biochemistry , dentistry
The present study shows the effect of interleukin‐1 β (IL‐1 β ) on some gene expressions and functions of fibroblastic cells (HPLF) derived from human periodontal ligament. HPLF were used at passages number 5 to 10. IL‐1 β increased DNA synthesis in both a dose‐ and an incubation time‐dependent manner. IL‐1 β in combination with tumor‐necrosis factor α or transforming growth factor β synergistically stimulated the DNA synthesis in the cells. Since many studies have shown that the c‐myc oncogene is involved in cell proliferation and differentiation, the effect of IL‐1 β on c‐myc messenger RNA (mRNA) level in HPLF was examined. IL‐1 β induced a marked c‐myc mRNA level in the cells at 90 minutes after initiation of the cytokine treatment. On the other hand, IL‐1 β significantly inhibited alkaline phosphatase (ALP) activity of the cells in a dose‐dependent manner. Also an inhibitory effect was observed on the liver/bone/kidney ALP mRNA level of the cells, and this inhibition by IL‐1 β was dose‐ and incubation time‐dependent. These results suggest that IL‐1 β is a regulatory cytokine involved in the regeneration of the human periodontal ligament.