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Measurement of relative avidity of antibodies reactive with Porphyromonas (Bacteroides) gingivalis in the sera of subjects having adult periodontitis
Author(s) -
Lopatin Dennis E.,
LaBelle Douglas,
Lee SeokWoo
Publication year - 1991
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1991.tb01641.x
Subject(s) - porphyromonas gingivalis , avidity , antibody , periodontitis , immunology , bacteroidaceae , bacteroides , microbiology and biotechnology , medicine , chemistry , biology , bacteria , genetics
Relative avidities of antibodies to Porphyromonas (Bacteroides) gingivalis in the sera of 15 patients having adult periodontitis and 15 healthy subjects were evaluated using an ammonium thiocyanate‐dissociated ELISA. Graded concentrations of ammonium thiocyanate were added to a single dilution of serum in order to dissociate low avidity antibody binding to P. gingivalis . The concentration of thiocyanate resulting in 50% reduction in binding (absorbance) was termed the ID 50 for that serum. When IgG‐class antibodies were examined, the ID 50 of anti‐ P. gingivalis antibodies in the sera of patients was significantly elevated (0.96M vs 0.71M; p<0.01, Student's t‐test). In contrast, when IgM‐class antibodies were examined no significant differences in ID 50 between patients and controls were found for P. gingivalis (0.54M vs 0.53M). While the ID 50 values of patient antibodies were found to be elevated relative to those of healthy controls, comparison with antibodies from rabbits immunized with P. gingivalis and with ID 50 values from other human studies suggests that adult humans, in general, produce very low‐avidity antibodies to P. gingivalis . It is suggested that the presence of low‐avidity antibodies contributes to the pathology associated with periodontal disease.