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CD11b expression on neutrophils in human crevicular fluid collected from clinically healthy gingivae
Author(s) -
Watanabe Keiko,
Hagen Karen L.,
Andersen Burton R.
Publication year - 1991
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1991.tb01631.x
Subject(s) - integrin alpha m , flow cytometry , downregulation and upregulation , granulocyte , chemistry , neutrophile , peripheral blood , cell , microbiology and biotechnology , immunology , andrology , medicine , endocrinology , biology , inflammation , biochemistry , gene
This study investigated the cell surface expression of CD11b (Mo 1, Mac‐1, CR 3) by neutrophils (PMNs) from human crevicular fluid (CF‐PMNs). CD11b expression on CF‐PMNs was compared, using flow cytometry, to that on peripheral blood PMNs (PB‐PMNs) isolated simultaneously from the same subjects. CD11b expression by CF‐PMNs was also compared to that on PBPMNs stimulated with formylmethionylleucylphenylalanine (fMLP) or crevicular fluid supernatant (cell‐free portion of CF diluted with buffer) (CFS). Crevicular PMNs consistently expressed more CD11b than unstimulated PB‐PMNs (p<0.001). The level of CD11b expressed on these CF‐PMNs was significantly greater than that expressed by PB‐PMNs stimulated with 10 −8 M fMLP (p<0.05). The level of CD11b surface expression on CF‐PMNs was not statistically different from that expressed on PB‐PMNs stimulated with CFS or 10 −6 M fMLP. However, CFS significantly stimulated upregulation of surface CDllb expression on PB‐PMNs relative to that achieved with 10 −8 M fMLP (0.01