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Interleukin‐2 stimulates osteoclastic activity; Increased acid production and radioactive calcium release
Author(s) -
Ries William L.,
Seeds Michael C.,
Key L. Lyndon
Publication year - 1989
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1989.tb01788.x
Subject(s) - calcium , chemistry , production (economics) , medicine , economics , macroeconomics
Recombinant human interleukin‐2 (IL‐2) was studied to determine effects on acid production by individual osteoclasts in situ on mouse calvarial bones. This analysis was performed using a microspectrofluorimetric technique to quantify acid production in individual cells. Radioactive calcium release was determined using calvarial bones in a standard tissue culture system. This allowed us to correlate changes in acid production with a measure of bone resorption. IL‐2 stimulated acid production and bone resorbing activity. Both effects were inhibited by calcitonin. No stimulation of bone resorption occurred when IL‐2‐containing test media was incubated with a specific anti‐IL‐2 antibody and ultrafiltered. Our data demonstrated a correlation between acid production and bone resorbing activity in mouse calvaria exposed to parathyroid hormone (PTH). The data obtained from cultured mouse calvaria exposed to IL‐2 demonstrated similar stimulatory effects to those seen during PTH exposure. These data suggest that calvaria exposed to IL‐2 in vitro have increased osteoclastic acid production corresponding with increased bone resorption.