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Regulation of human gingival fibroblast growth and synthetic activity by cyclosporine‐A in vitro
Author(s) -
Bartold P. Mark
Publication year - 1989
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1989.tb00876.x
Subject(s) - foreskin , fibroblast , stimulation , in vitro , in vivo , dna synthesis , endocrinology , medicine , lipopolysaccharide , fetus , biology , andrology , chemistry , cell culture , pregnancy , biochemistry , genetics , microbiology and biotechnology
Gingival overgrowth is an adverse side‐effect seen in a proportion of patients taking cyclosporine‐A which indicates that cyclosporine‐A may modulate the activities of cells other than T lymphocytes. Therefore, the effect of cyclosporine on human gingival fibroblasts has been studied in vitro. Cyclosporine‐A was found to stimulate DNA synthesis and the proliferative activity of these cells with maximal stimulation noted at a concentration of 10 –9 g/ml. Although this stimulation was most noticeable in the presence of 10% fetal calf serum, proliferation still occurred in serum‐free medium. In the presence of lipopolysaccharide, at a concentration which normally inhibits ingival fibroblast proliferation, cyclosporine retained its capacity to stimulate proliferative activity. Fibroblasts isolated from overgrown gingival tissue responded to a greater extent than those isolated from a healthy site from the same individual. This stimulatory effect was not restricted to gingival fibroblasts, since human foreskin fibroblasts responded in a similar fashion. Cyclosporine‐A did not significantly alter protein or proteoglycan production by these cells. These responses are considered to reflect the in vivo response of gingival overgrowth in patients taking cyclosporine‐A. The reversal of lipopolysaccharide inhibition of gingival fibroblast proliferation by cyclosporine‐A may explain, in part, why gingival overgrowth is most prominent in areas of heavy dental plaque accumulation.