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Bactericidal concentrations of chlorhexidine‐digluconate, amine fluoride gel and stannous fluoride gel for subgingival bacteria tested in serum at short contact times
Author(s) -
Oosterwaal P. J. M.,
Mikx F. H. M.,
Brink M. E.,
Renggli H. H.
Publication year - 1989
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1989.tb00871.x
Subject(s) - fusobacterium nucleatum , chemistry , fluoride , microbiology and biotechnology , chlorhexidine , minimum bactericidal concentration , minimum inhibitory concentration , amoxicillin , actinobacillus , nuclear chemistry , chromatography , antibiotics , porphyromonas gingivalis , dentistry , medicine , biochemistry , periodontitis , inorganic chemistry , biology
In vitro inhibitory and bactericidal concentrations in serum of chlorhexidine‐digluconate, amine fluoride gel, stannous fluoride gel, stannous fluoride, metronidazole and amoxicillin were determined against Bacteroides gingivalis , Bacteroides intermedius, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans and Capnocytophaga sputigena . The minimal inhibitory concentration was assessed by the agar dilution technique. The killing curves and minimal bactericidal concentration of the antimicrobial agents in inactivated bovine serum were determined after 5, 10, 20 and 60 minutes contact time. The minimal inhibitory concentration varied amongst the tested bacteria. A concentration of 128 μg/ml chlorhexidine digluconate, 20 mg/ml amine fluoride gel, 1 mg/ml stannous fluoride, 128 μg/ml metronidazole and 4 μg/ml amoxicillin inhibited the growth of the tested species. The minimal bactericidal concentration in serum for B. gingivalis, B. intermedius, F. nucleatum, A. actinomycetemcomilans and C. sputigena after 10 min contact time was 5 mg/ml for chlorhexidine digluconate and 100 mg/ml for amine fluoride gel. A concentration of 200 mg/ml stannous fluoride gel in serum was bactericidal for the tested species after 10 min contact time, with exception of F. nucleatum .