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Electron immunolocalization of type IV collagen, laminin and fibronectin synthesized by multilayered cells cultured from human oral epithelium
Author(s) -
Poly H.,
Couble M. L.,
Hartmann D. J.,
Faure M.,
Magloire H.
Publication year - 1988
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/j.1600-0765.1988.tb01368.x
Subject(s) - laminin , fibronectin , keratin , type iv collagen , extracellular matrix , epithelium , microbiology and biotechnology , basal (medicine) , biology , matrix (chemical analysis) , chemistry , pathology , endocrinology , medicine , paleontology , genetics , chromatography , insulin
Human palatal epithelial cells were grown on lethally irradiated 3T3 fibroblasts. Under these conditions, epithelial cultures become stratified. The basal cells have a cuboïdal shape and suprabasal layers are flattened. The reconstituted epithelium exhibits markers of differentiation: intracytoplasmic bundles of keratin‐like filaments and abundant desmosmes present in every layer. Type IV collagen fibronectin and laminin are synthesized by these cultures. Basal cells grip the culture substratum via an extracellular matrix made up of type IV collagen, laminin and fibronectin.